ARG52331
anti-MeCP2 phospho (Ser80) antibody
anti-MeCP2 phospho (Ser80) antibody for Immunohistochemistry,Western blot and Human,Mouse,Rat
Gene Regulation antibody; Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MeCP2 phospho (Ser80) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MeCP2 |
| Antigen Species | Human |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser80 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | MRXSL; RS; MeCp2; Methyl-CpG-binding protein 2; MeCp-2 protein; RTT; AUTSX3; RTS; MRX79; PPMX; MRXS13; MRX16 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~75 kDa MECP2 protein phosphorylated at Ser80 in Western blots of human, Rat and mouse brain extracts. The antibody has also been used successfully for immunohistochemistry on mouse brain sections. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Serum |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MECP2 |
| Gene Full Name | methyl CpG binding protein 2 |
| Background | MECP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MECP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Defects in MECP2 are the cause of Rett syndrome (RTT). RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser80. Phosphorylation and dephosphorylation of this site may be involved in modulating the dynamic function of MECP2 in neurons transiting between resting and active states within neural circuits that underlie behaviors. (Tao et al., 2009) |
| Research Area | Gene Regulation antibody; Neuroscience antibody |
| Calculated MW | 52 kDa |
| PTM | Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. |
