ARG59014

anti-Methylmalonyl-CoA mutase antibody

anti-Methylmalonyl-CoA mutase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Methylmalonyl-CoA mutase
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Methylmalonyl-CoA mutase
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 33-65 of Human Methylmalonyl-CoA mutase (LHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPE).
Conjugation Un-conjugated
Alternate Names Methylmalonyl-CoA isomerase; MCM; EC 5.4.99.2; Methylmalonyl-CoA mutase, mitochondrial

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: By heat mediation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17850 Mouse MUT

GeneID: 4594 Human MUT

Swiss-port # P16332 Mouse Methylmalonyl-CoA mutase, mitochondrial

Swiss-port # P22033 Human Methylmalonyl-CoA mutase, mitochondrial

Gene Symbol MUT
Gene Full Name methylmalonyl CoA mutase
Background This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq, Jul 2008]
Function Involved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species. [UniProt]
Cellular Localization Mitochondrion matrix. [UniProt]
Calculated MW 83 kDa