ARG64655

anti-Monoglyceride Lipase antibody

anti-Monoglyceride Lipase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes Monoglyceride Lipase
Tested Reactivity Hu, Ms
Predict Reactivity Cow, Dog
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize both reported isoforms (NP_009214.1; NP_001003794.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Monoglyceride Lipase
Antigen Species Human
Immunogen C-QDLPHLVNADGQY
Conjugation Un-conjugated
Alternate Names Lysophospholipase-like; Monoacylglycerol lipase; EC 3.1.1.23; HUK5; Lysophospholipase homolog; MAGL; HU-K5; Monoglyceride lipase; MGL

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.5 - 1.5 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11343 Human MGLL

GeneID: 23945 Mouse MGLL

Swiss-port # O35678 Mouse Monoglyceride lipase

Swiss-port # Q99685 Human Monoglyceride lipase

Background This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 33 kDa