ARG55970
anti-Mucin 5AC antibody [45M1]
anti-Mucin 5AC antibody [45M1] for IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [45M1] recognizes Mucin 5AC |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Species Does Not React With | Cow |
| Tested Application | IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 45M1 |
| Isotype | IgG1, kappa |
| Target Name | Mucin 5AC |
| Antigen Species | Human |
| Immunogen | Mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient. |
| Conjugation | Un-conjugated |
| Alternate Names | leB; Major airway glycoprotein; Lewis B blood group antigen; Tracheobronchial mucin; mucin; Mucin-5AC; LeB; MUC5; TBM; Gastric mucin; MUC-5AC; Mucin-5 subtype AC, tracheobronchial |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MUC5AC |
| Gene Full Name | mucin 5AC, oligomeric mucus/gel-forming |
| Function | Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system. [UniProt] |
| Cellular Localization | Cytoplasmic |
| Highlight | Related products: Mucin 5AC antibodies; Anti-Mouse IgG secondary antibodies; Related news: Wnt / beta-catenin signaling for gastric fundus specification More than a biomarker, CA19-9 is a therapeutic target of pancreatic cancer |
| Calculated MW | 586 kDa |
| PTM | C-, O- and N-glycosylated. O-glycosylated on the Thr-/Ser-rich tandem repeats. C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis. Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide. |
