ARG52343
anti-Munc18 phospho (Ser 515) antibody
anti-Munc18 phospho (Ser 515) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Rat
Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Munc18 phospho (Ser 515) |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms, Bov, Chk, Dog, NHuPrm, Xenopus laevis |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Munc18 |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser515 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | Protein unc-18 homolog B; UNC18-2; Unc-18B; MUNC18-2; Unc18-2; Protein unc-18 homolog 2; UNC18B; FHL5; pp10122; Hunc18b; Syntaxin-binding protein 2 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~65k Munc-18 protein phosphorylated at Ser513 . Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | STXBP2 |
| Gene Full Name | syntaxin binding protein 2 |
| Background | Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Unc-18 is thought to be regulated by PKC phosphorylation of Ser515 in C. Elegans (Sassa et al., 1996). Ser513 is the mammalian homologue of Ser515 in C. Elegans. |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 66 kDa |
