ARG56986
anti-Myosin Light Chain 2 antibody [3B2]
anti-Myosin Light Chain 2 antibody [3B2] for Flow cytometry,Western blot and Human,Rat
Overview
| Product Description | Mouse Monoclonal antibody [3B2] recognizes Myosin Light Chain 2 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3B2 |
| Isotype | IgG2b, kappa |
| Target Name | Myosin Light Chain 2 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-166 of Human Myosin Light Chain 2. |
| Conjugation | Un-conjugated |
| Alternate Names | MLC2; Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; CMH10; MLC-2; MLC-2v |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08733 Rat Myosin regulatory light chain 2, ventricular/cardiac muscle isoform Swiss-port # P10916 Human Myosin regulatory light chain 2, ventricular/cardiac muscle isoform |
|---|---|
| Gene Symbol | MYL2 |
| Gene Full Name | myosin, light chain 2, regulatory, cardiac, slow |
| Background | Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008] |
| Calculated MW | 19 kDa |
| PTM | N-terminus is methylated by METTL11A/NTM1. Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function (By similarity). Dephosphorylated by PPP1CB complexed to PPP1R12B (By similarity). The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (By similarity). |
