ARG51616

anti-Myosin Light Chain 9 phospho (Ser19) antibody

anti-Myosin Light Chain 9 phospho (Ser19) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Myosin Light Chain 9 phospho (Ser19)
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Myosin Light Chain 9
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 19 (A-T-S(p)-N-V) derived from Human Myosin Light Chain 9. (NP_006088.2)
Conjugation Un-conjugated
Alternate Names LC20; Myosin RLC; 20 kDa myosin light chain; Myosin regulatory light chain 2, smooth muscle isoform; MYRL2; MLC2; Myosin regulatory light chain MRLC1; Myosin regulatory light chain 9; Myosin regulatory light polypeptide 9; MRLC1; MLC-2C

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10398 Human MYL9

GeneID: 296313 Rat MYL9

GeneID: 98932 Mouse MYL9

Gene Symbol MYL9
Gene Full Name myosin, light chain 9, regulatory
Background Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion
Research Area Signaling Transduction antibody
Calculated MW 20 kDa
PTM Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.