ARG59943

anti-Myosin light chain 3 antibody

anti-Myosin light chain 3 antibody for Western blot,ICC/IF and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes Myosin light chain 3
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Myosin light chain 3
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-195 of Human Myosin light chain 3 (NP_000249.1).
Conjugation Un-conjugated
Alternate Names MLC1SB; Ventricular/slow twitch myosin alkali light chain; VLC1; CMH8; Myosin light chain 3; Myosin light chain 1, slow-twitch muscle B/ventricular isoform; MLC1V; CMLC1; Cardiac myosin light chain 1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse lung and HepG2
Observed Size 23 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17897 Mouse MYL3

GeneID: 4634 Human MYL3

Swiss-port # P08590 Human Myosin light chain 3

Swiss-port # P09542 Mouse Myosin light chain 3

Gene Symbol MYL3
Gene Full Name myosin, light chain 3, alkali; ventricular, skeletal, slow
Background MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
Function Regulatory light chain of myosin. Does not bind calcium. [UniProt]
Calculated MW 22 kDa
PTM The N-terminus is blocked.

N-terminus is methylated by METTL11A/NTM1. [UniProt]