ARG42577

anti-NAA10 / ARD1A antibody

anti-NAA10 / ARD1A antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NAA10 / ARD1A
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NAA10 / ARD1A
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 161-235 of Human NAA10 / ARD1A (NP_003482.1).
Conjugation Un-conjugated
Alternate Names N-alpha-acetyltransferase 10; ARD1P; N-terminal acetyltransferase complex ARD1 subunit homolog A; DXS707; EC 2.3.1.88; ARD1; NatA catalytic subunit Naa10; EC 2.3.1.-; ARD1A; TE2; MCOPS1; NATD

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56292 Mouse NAA10

GeneID: 8260 Human NAA10

Swiss-port # P41227 Human N-alpha-acetyltransferase 10

Swiss-port # Q9QY36 Mouse N-alpha-acetyltransferase 10

Gene Symbol NAA10
Gene Full Name N(alpha)-acetyltransferase 10, NatA catalytic subunit
Background N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Function Catalytic subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity (PubMed:15496142, PubMed:19826488, PubMed:19420222, PubMed:20145209, PubMed:27708256, PubMed:25489052, PubMed:29754825). Acetylates amino termini that are devoid of initiator methionine (PubMed:19420222). The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation (PubMed:12464182). Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration (PubMed:19826488). Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development (PubMed:20145209). Acetylates HSPA1A and HSPA1B at 'Lys-77' which enhances its chaperone activity and leads to preferential binding to co-chaperone HOPX (PubMed:27708256). Acetylates HIST1H4A (PubMed:29754825). Acts as a negative regulator of sister chromatid cohesion during mitosis (PubMed:27422821). [UniProt]
Cellular Localization Cytoplasm. Nucleus. Note=Also present in the free cytosolic and cytoskeleton-bound polysomes. [UniProt]
Calculated MW 26 kDa
PTM Cleaved by caspases during apoptosis.

Phosphorylation by IKBKB/IKKB at Ser-209 promotes its proteasome-mediated degradation.

Autoacetylated at Lys-136 which stimulates its catalytic activity. [UniProt]