ARG42576

anti-NAA60 / NAT15 antibody

anti-NAA60 / NAT15 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NAA60 / NAT15
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NAA60 / NAT15
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-242 of Human NAA60 / NAT15 (NP_079121.1).
Conjugation Un-conjugated
Alternate Names EC 2.3.1.48; N-alpha-acetyltransferase 60; Histone acetyltransferase type B protein 4; N-acetyltransferase 15; NatF catalytic subunit; HAT4; EC 2.3.1.88; NAT15

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse brain
Observed Size ~ 32 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 363545 Rat NAA60

GeneID: 74763 Mouse NAA60

GeneID: 79903 Human NAA60

Gene Symbol NAA60
Gene Full Name N(alpha)-acetyltransferase 60, NatF catalytic subunit
Background This gene encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located in a differentially methylated region (DMR) and undergoes imprinting; transcript variants originating from this position are expressed from the maternal allele. [provided by RefSeq, Nov 2015]
Function N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues of the transmembrane proteins, with a strong preference for N-termini facing the cytosol (PubMed:25732826). Displays N-terminal acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met (PubMed:21750686, PubMed:25732826, PubMed:27550639, PubMed:27320834). Required for normal chromosomal segregation during anaphase (PubMed:21750686). May also show histone acetyltransferase activity; such results are however unclear in vivo and would require additional experimental evidences (PubMed:21981917). [UniProt]
Cellular Localization Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Probably forms a intramembrane hairpin-like structure in the membrane. [UniProt]
Calculated MW 27 kDa
PTM Acetylated: autoacetylation is required for optimal acetyltransferase activity. [UniProt]