ARG59180
anti-NAE1 / APPBP1 antibody
anti-NAE1 / APPBP1 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NAE1 / APPBP1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NAE1 / APPBP1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide within aa. 430-459 of Human NAE1 / APPBP1. |
| Conjugation | Un-conjugated |
| Alternate Names | APP-BP1; APPBP1; A-116A10.1; Amyloid protein-binding protein 1; HPP1; Amyloid beta precursor protein-binding protein 1, 59 kDa; NEDD8-activating enzyme E1 regulatory subunit; ula-1; Proto-oncogene protein 1 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13564 Human NEDD8-activating enzyme E1 regulatory subunit |
|---|---|
| Gene Symbol | NAE1 |
| Gene Full Name | NEDD8 activating enzyme E1 subunit 1 |
| Background | The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. [UniProt] |
| Cellular Localization | Cell membrane. Note=Colocalizes with APP in lipid rafts. [UniProt] |
| Calculated MW | 60 kDa |
| PTM | Ubiquitinated by TRIP12, leading to its degradation by the proteasome. [UniProt] |
