ARG56612

anti-NANOG antibody

anti-NANOG antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Cow,Dog,Pig

Overview

Product Description Rabbit Polyclonal antibody recognizes NANOG
Tested Reactivity Hu, Ms, Cow, Dog, Pig
Tested Application ELISA, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NANOG
Antigen Species Human
Immunogen E.coli derived Recombinant Human Nanog.
(SVDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDV)
Conjugation Un-conjugated
Alternate Names Homeobox transcription factor Nanog; Homeobox protein NANOG; hNanog

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56727 as a detection antibody
IHC-P0.5 µg/ml
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 71950 Mouse NANOG

GeneID: 79923 Human NANOG

Swiss-port # Q80Z64 Mouse Homeobox protein NANOG

Swiss-port # Q9H9S0 Human Homeobox protein NANOG

Gene Symbol NANOG
Gene Full Name Nanog homeobox
Background The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Function Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. [UniProt]
Calculated MW 35 kDa