ARG64265
anti-NCF4 / P40PHOX antibody
anti-NCF4 / P40PHOX antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
Product Description | Goat Polyclonal antibody recognizes NCF4 / P40PHOX |
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Tested Reactivity | Hu |
Tested Application | IHC-P, WB |
Specificity | This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1). |
Host | Goat |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | NCF4 / P40PHOX |
Antigen Species | Human |
Immunogen | C-KDFPEEDDPTN |
Conjugation | Un-conjugated |
Alternate Names | p40phox; SH3PXD4; Neutrophil NADPH oxidase factor 4; Neutrophil cytosol factor 4; P40PHOX; SH3 and PX domain-containing protein 4; p40-phox; NCF; NCF-4 |
Application Instructions
Application Suggestion |
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Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
Preservative | 0.02% Sodium azide |
Stabilizer | 0.5% BSA |
Concentration | 0.5 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Background | The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
Calculated MW | 39 kDa |