ARG64265

anti-NCF4 / P40PHOX antibody

anti-NCF4 / P40PHOX antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes NCF4 / P40PHOX
Tested Reactivity Hu
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name NCF4 / P40PHOX
Antigen Species Human
Immunogen C-KDFPEEDDPTN
Conjugation Un-conjugated
Alternate Names p40phox; SH3PXD4; Neutrophil NADPH oxidase factor 4; Neutrophil cytosol factor 4; P40PHOX; SH3 and PX domain-containing protein 4; p40-phox; NCF; NCF-4

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2.5 µg/ml
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4689 Human NCF4

Swiss-port # Q15080 Human Neutrophil cytosol factor 4

Background The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 39 kDa