ARG22546

anti-NCR1 antibody [AKS1]

anti-NCR1 antibody [AKS1] for Flow cytometry,ICC/IF,Western blot and Bovine

Overview

Product Description Mouse Monoclonal antibody [AKS1] recognizes NCR1
This antibody recognizes bovine CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1. CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor. CD335 is expressed by human natural killer cells (Sivori et al. 1997). The bovine homologue is expressed on bovine NK cells (Storset et al. 2004) and no expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.Clone AKS1 is reported to activate lysis of FcgammaR-expressing cell line P815, by IL-2 activated NKp46+ cells (Storset et al. 2004).
Tested Reactivity Bov
Tested Application FACS, ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone AKS1
Isotype IgG1
Target Name NCR1
Antigen Species Bovine
Immunogen Fusion protein consisting of the extracellular region of bovine CD335.
Conjugation Un-conjugated
Alternate Names CD antigen CD335; Natural killer cell p46-related protein; Lymphocyte antigen 94 homolog; Natural cytotoxicity triggering receptor 1; hNKp46; NKP46; NKp46; NK cell-activating receptor; LY94; CD335; NK-p46

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat
ICC/IFAssay-dependent
WBAssay-dependent
Application Note FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 369024 Bovine NCR1

Swiss-port # Q863H2 Bovine Natural cytotoxicity triggering receptor 1

Gene Symbol NCR1
Gene Full Name natural cytotoxicity triggering receptor 1
Function Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. [UniProt]
Calculated MW 34 kDa
PTM N-glycosylated.
O-glycosylated.