ARG43419
anti-NDR1 phospho (Thr444) + NDR2 phospho (Thr442) antibody
anti-NDR1 phospho (Thr444) + NDR2 phospho (Thr442) antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDR1 phospho (Thr444) + NDR2 phospho (Thr442) |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDR1 + NDR2 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated phosphospecific peptide around Thr444 of Human NDR1 or around Thr442 of Human NDR2. |
| Conjugation | Un-conjugated |
| Alternate Names | NDR1: Nuclear Dbf2-related kinase 1; NDR1 protein kinase; Serine/threonine-protein kinase 38; EC 2.7.11.1; NDR NDR2: Serine/threonine-protein kinase 38-like; EC 2.7.11.1; Nuclear Dbf2-related kinase 2; NDR2 protein kinase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | HepG2 + EGF | ||||
| Observed Size | ~ 54 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with phospho-specific peptide and the non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. |
| Buffer | PBS (pH 7.4, without Mg2+ and Ca2+), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q15208 Human Serine/threonine-protein kinase 38 |
|---|---|
| Gene Symbol | STK38; STK38L |
| Gene Full Name | serine/threonine kinase 38 serine/threonine kinase 38 like |
| Background | NDR1: This gene encodes a member of the AGC serine/threonine kinase family of proteins. The kinase activity of this protein is regulated by autophosphorylation and phosphorylation by other upstream kinases. This protein has been shown to function in the cell cycle and apoptosis. This protein has also been found to regulate the protein stability and transcriptional activity of the MYC oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015] |
| Function | NDR1: Negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its non-phosphorylated form and inhibits autophosphorylation of MAP3K2. [UniProt] NDR2: Involved in the regulation of structural processes in differentiating and mature neuronal cells. [UniProt] |
| Cellular Localization | NDR1: Nucleus. Cytoplasm. [UniProt] NDR2: Cytoplasm. Cytoplasm, cytoskeleton. Membrane. Note=Associated with the actin cytoskeleton. Co-localizes with STK24/MST3 in the membrane. [UniProt] |
| Calculated MW | NDR1 and NDR2: 54 kDa |
| PTM | NDR1: ISGylated; Phosphorylated by STK3/MST2 and this is enhanced by MOBKL1B. [UniProt] |
