ARG40439

anti-NDUFB2 antibody

anti-NDUFB2 antibody for Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes NDUFB2
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NDUFB2
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 34-105 of Human NDUFB2 (NP_004537.1).
Conjugation Un-conjugated
Alternate Names CI-AGGG; AGGG; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial; Complex I-AGGG; NADH-ubiquinone oxidoreductase AGGG subunit

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse liver
Observed Size 12 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4708 Human NDUFB2

GeneID: 68198 Mouse NDUFB2

Swiss-port # O95178 Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial

Swiss-port # Q9CPU2 Mouse NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial

Gene Symbol NDUFB2
Gene Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa
Background The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays a important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Hydropathy analysis revealed that this subunit and 4 other subunits have an overall hydrophilic pattern, even though they are found within the hydrophobic protein (HP) fraction of complex I. [provided by RefSeq, Jul 2008]
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt]
Cellular Localization Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. [UniProt]
Calculated MW 12 kDa