ARG43596

anti-NDUFB8 antibody

anti-NDUFB8 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NDUFB8
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NDUFB8
Antigen Species Human
Immunogen Synthetic peptide corresponding to a sequence of Human NDUFB8.
Conjugation Un-conjugated
Protein Full Name NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
Alternate Names ASHI; CI-ASHI; MC1DN32; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; Complex I-ASHI; CI-ASHI; NADH-ubiquinone oxidoreductase ASHI subunit

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:20 - 1:30
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa cell
Observed Size ~ 20 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4714 Human NDUFB8

GeneID: 67264 Mouse NDUFB8

Swiss-port # O95169 Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

Swiss-port # Q9D6J5 Mouse NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

Gene Symbol NDUFB8
Gene Full Name NADH:ubiquinone oxidoreductase subunit B8
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt]
Cellular Localization Membrane, Mitochondrion, Mitochondrion inner membrane
Calculated MW 21.7 kDa