ARG42814

anti-NDUFB9 antibody

anti-NDUFB9 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Flow cytometry,Immunoprecipitation and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NDUFB9
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NDUFB9
Antigen Species Human
Immunogen Recombinant protein of Human NDUFB9.
Conjugation Un-conjugated
Alternate Names NADH-ubiquinone oxidoreductase B22 subunit; CI-B22; LYR motif-containing protein 3; B22; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; LYRM3; UQOR22; Complex I-B22

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50
IHC-P1:50
IP1:20
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat brain
Observed Size ~ 23 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 nM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4715 Human NDUFB9

GeneID: 66218 Mouse NDUFB9

Swiss-port # Q9CQJ8 Mouse NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

Swiss-port # Q9Y6M9 Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

Gene Symbol NDUFB9
Gene Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa
Background The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt]
Cellular Localization Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. [UniProt]
Calculated MW 22 kDa