ARG65261

anti-NDUFS1 antibody

anti-NDUFS1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes NDUFS1
Tested Reactivity Hu, Ms
Predict Reactivity Cow, Rat, Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name NDUFS1
Antigen Species Human
Immunogen C-TEKSATYVNTEGR
Conjugation Un-conjugated
Alternate Names CI-75k; EC 1.6.5.3; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; CI-75Kd; EC 1.6.99.3; PRO1304; Complex I-75kD; CI-75kD

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 227197 Mouse NDUFS1

GeneID: 4719 Human NDUFS1

Swiss-port # P28331 Human NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Swiss-port # Q91VD9 Mouse NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Background The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
Research Area Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 79 kDa