ARG65574

anti-NDUFS2 antibody

anti-NDUFS2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes NDUFS2
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NDUFS2
Antigen Species Human
Immunogen Synthetic peptide of human NDUFS2
Conjugation Un-conjugated
Alternate Names Complex I-49kD; CI-49kD; CI-49; NADH-ubiquinone oxidoreductase 49 kDa subunit; EC 1.6.99.3; NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; EC 1.6.5.3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P50-200
WB500-2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse heart and brain tissue, Jurkat cell

Properties

Form Liquid
Purification Purified by antigen-affinity chromatography.
Buffer 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol
Preservative 0.05% Sodium azide
Stabilizer 40% Glycerol
Concentration 3.1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 226646 Mouse NDUFS2

GeneID: 4720 Human NDUFS2

Swiss-port # O75306 Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Swiss-port # Q91WD5 Mouse NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Gene Symbol NDUFS2
Gene Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
Background The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). [UniProt]
Highlight Related products:
NDUFS2 antibodies; Anti-Rabbit IgG secondary antibodies;
Related poster download:
The Structure & Functions of Mitochondria.pdf
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 53 kDa
PTM Dimethylation at Arg-118 by NDUFAF7 takes place after NDUFS2 assembles into the complex I, leading to stabilize the early intermediate complex (PubMed:24089531, PubMed:24838397).