ARG65574
anti-NDUFS2 antibody
anti-NDUFS2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
Product Description | Rabbit Polyclonal antibody recognizes NDUFS2 |
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Tested Reactivity | Hu, Ms |
Tested Application | IHC-P, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | NDUFS2 |
Antigen Species | Human |
Immunogen | Synthetic peptide of human NDUFS2 |
Conjugation | Un-conjugated |
Alternate Names | Complex I-49kD; CI-49kD; CI-49; NADH-ubiquinone oxidoreductase 49 kDa subunit; EC 1.6.99.3; NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; EC 1.6.5.3 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
Positive Control | Mouse heart and brain tissue, Jurkat cell |
Properties
Form | Liquid |
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Purification | Purified by antigen-affinity chromatography. |
Buffer | 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
Preservative | 0.05% Sodium azide |
Stabilizer | 40% Glycerol |
Concentration | 3.1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # O75306 Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial Swiss-port # Q91WD5 Mouse NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial |
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Gene Symbol | NDUFS2 |
Gene Full Name | NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) |
Background | The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Function | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). [UniProt] |
Highlight | Related products: NDUFS2 antibodies; Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
Calculated MW | 53 kDa |
PTM | Dimethylation at Arg-118 by NDUFAF7 takes place after NDUFS2 assembles into the complex I, leading to stabilize the early intermediate complex (PubMed:24089531, PubMed:24838397). |