ARG55793

anti-NEDD4 antibody

anti-NEDD4 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes NEDD4
Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NEDD4
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 1288-1319 (C-terminus) of Human NEDD4.
Conjugation Un-conjugated
Alternate Names NEDD-4; EC 6.3.2.-; RPF1; E3 ubiquitin-protein ligase NEDD4; NEDD4-1; Cell proliferation-inducing gene 53 protein; Neural precursor cell expressed developmentally down-regulated protein 4

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-P1:50 - 1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control NIH/3T3

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17999 Mouse NEDD4

GeneID: 4734 Human NEDD4

Swiss-port # P46934 Human E3 ubiquitin-protein ligase NEDD4

Swiss-port # P46935 Mouse E3 ubiquitin-protein ligase NEDD4

Gene Symbol NEDD4
Gene Full Name neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. The direct link between NEDD4 and PTEN regulation through polyubiquitination is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. [UniProt]
Cellular Localization Cytoplasm. Cell membrane; Peripheral membrane protein Note=Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1. 
Calculated MW 149 kDa
PTM Auto-ubiquitinated.