ARG40446
anti-NEK3 antibody
anti-NEK3 antibody for Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NEK3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NEK3 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 267-506 of Human NEK3 (NP_689933.1). |
| Conjugation | Un-conjugated |
| Alternate Names | HSPK36; Never in mitosis A-related kinase 3; HSPK 36; Serine/threonine-protein kinase Nek3; EC 2.7.11.1; NimA-related protein kinase 3 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | HeLa | ||||
| Observed Size | 55 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P51956 Human Serine/threonine-protein kinase Nek3 Swiss-port # Q9R0A5 Mouse Serine/threonine-protein kinase Nek3 |
|---|---|
| Gene Symbol | NEK3 |
| Gene Full Name | NIMA-related kinase 3 |
| Background | This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009] |
| Function | Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2. [UniProt] |
| Cellular Localization | Cytoplasm. Cell projection, axon. [UniProt] |
| Calculated MW | 58 kDa |
| PTM | Phosphorylation at Thr-479 regulates its catalytic activity. [UniProt] |
