ARG40442

anti-NEU1 / Neuraminidase antibody

anti-NEU1 / Neuraminidase antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NEU1 / Neuraminidase
Tested Reactivity Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NEU1 / Neuraminidase
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 156-415 of Human NEU1 (NP_000425.1).
Conjugation Un-conjugated
Alternate Names NANH; Acetylneuraminyl hydrolase; SIAL1; Lysosomal sialidase; EC 3.2.1.18; N-acetyl-alpha-neuraminidase 1; Sialidase-1; NEU; G9 sialidase

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse liver
Observed Size 48 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18010 Mouse NEU1

Swiss-port # O35657 Mouse Sialidase-1

Gene Symbol NEU1
Gene Full Name sialidase 1 (lysosomal sialidase)
Background The protein encoded by this gene is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter is also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red spot-myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. [provided by RefSeq, Jul 2008]
Function Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage. [UniProt]
Cellular Localization Lysosome membrane; Peripheral membrane protein; Lumenal side. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Lysosome. Note=Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles. [UniProt]
Calculated MW 45 kDa
PTM N-glycosylated.

Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane. [UniProt]