ARG23706

anti-NFIA antibody

anti-NFIA antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NFIA
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NFIA
Antigen Species Human
Immunogen Synthetic peptide around aa. 180-224 of Human NFIA. (AYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFS)
Conjugation Un-conjugated
Alternate Names Nuclear factor 1/A; Nuclear factor 1 A-type; TGGCA-binding protein; NF-I/A; NF1-A; CTF; CCAAT-box-binding transcription factor; NFI-L; Nuclear factor I/A; NFI-A

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P1 - 2 µg/ml
WB0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.025% Sodium azide and 2.5% BSA.
Preservative 0.025% Sodium azide
Stabilizer 2.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18027 Mouse NFIA

GeneID: 25492 Rat NFIA

GeneID: 4774 Human NFIA

Gene Symbol NFIA
Gene Full Name nuclear factor I/A
Background This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Function Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. [UniProt]
Calculated MW 56 kDa (unmodified); 60-70 kDa (phosphorylated)