ARG66549

anti-NFYC antibody

anti-NFYC antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes NFYC
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NFYC
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of Human NFYC.
Conjugation Un-conjugated
Alternate Names HAP5; NF-YC; Nuclear transcription factor Y subunit gamma; Nuclear transcription factor Y subunit C; CBF-C; HSM; CBFC; Transactivator HSM-1/2; CAAT box DNA-binding protein subunit C; H1TF2A

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
WB1:500 - 1:1000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 50 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
Preservative 0.01% Sodium azide
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18046 Mouse NFYC

GeneID: 4802 Human NFYC

Swiss-port # P70353 Mouse Nuclear transcription factor Y subunit gamma

Swiss-port # Q13952 Human Nuclear transcription factor Y subunit gamma

Gene Symbol NFYC
Gene Full Name nuclear transcription factor Y, gamma
Background This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
Function Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 50 kDa