ARG45066
anti-NGEF antibody
anti-NGEF antibody for ICC/IF,Western blot and Chicken,Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NGEF |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Chk |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NGEF |
| Antigen Species | Mouse |
| Immunogen | Ephexin-1 synthetic peptide corresponds to amino acids in the C-terminal region of mouse ephexin-1. This sequence has significant homology to the same region in human and rat ephexin-1, and has low homology to other known proteins. |
| Conjugation | Un-conjugated |
| Alternate Names | NGEF; Neuronal Guanine Nucleotide Exchange Factor; ARHGEF27; Eph-Interacting Exchange Protein; Ephexin-1; Ephexin1; Ephexin |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antigen Affinity Purified. |
| Buffer | PBS, 0.05% NaN3, 50% Glycerol and 0.1 % BSA. |
| Stabilizer | 50% Glycerol and 0.1 % BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NGEF |
| Gene Full Name | Neuronal Guanine Nucleotide Exchange Factor |
| Background | Enables guanyl-nucleotide exchange factor activity. Predicted to be involved in several processes, including activation of GTPase activity; ephrin receptor signaling pathway; and negative regulation of dendritic spine morphogenesis. Predicted to be located in cytosol. Predicted to be active in glutamatergic synapse. [provided by Alliance of Genome Resources, Apr 2022] |
| Function | Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension |
| Cellular Localization | Cell projection, Cytoplasm, Membrane |
| Calculated MW | 82 kDa |
| PTM | Phosphoprotein |
