ARG20457
anti-NIK antibody
anti-NIK antibody for Western blot and Human,Mouse,Rat,Dog,Hamster
Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NIK |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Dog, Hm |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NIK |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around 927 aa of Human NIK protein (NP_003945.2) |
| Conjugation | Un-conjugated |
| Alternate Names | NIK; HSNIK; FTDCR1B; HS; EC 2.7.11.25; HsNIK; Serine/threonine-protein kinase NIK; NF-kappa-beta-inducing kinase; Mitogen-activated protein kinase kinase kinase 14 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2), 50% Glycerol, 1% BSA and 0.02% Thimerosal |
| Preservative | 0.02% Thimerosal |
| Stabilizer | 50% Glycerol, 1% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99558 Human Mitogen-activated protein kinase kinase kinase 14 Swiss-port # Q9WUL6 Mouse Mitogen-activated protein kinase kinase kinase 14 |
|---|---|
| Gene Symbol | MAP3K14 |
| Gene Full Name | mitogen-activated protein kinase kinase kinase 14 |
| Background | NIK (NF-kB-inducing kinase) is a member of the MAP kinase kinase kinase family that binds TRAF2 and stimulates NF-kB activity. NIK was initially isolated from a human B cell cDNA library and contains 795 amino acids with an apparent molecular weight of slightly more than 97 kDa on SDS gel. NIK is a serine/threonine kinase and its kinase activity contributes to IkB phosphorylation. The carboxyl terminal segment of NIK binds TRAF2. A mutant NIK with intact carboxyl terminus but without the two lysine residues at its catalytic domain serves as a dominant-negative inhibitor for NF-kB activation. NIK also interacts with TRAF6 and mediates IL-1-induced NF-kB activation. |
| Highlight | Related products: NIK antibodies; Anti-Rabbit IgG secondary antibodies; Related poster download: The NF-kappa B Pathways.pdf |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody |
| Calculated MW | 104 kDa |
| PTM | Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization. Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. |
