ARG54322
anti-NIK antibody
anti-NIK antibody for ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NIK |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NIK |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa 931-947 of human NIK (accession no. Q99558). |
| Conjugation | Un-conjugated |
| Alternate Names | NIK; HSNIK; FTDCR1B; HS; EC 2.7.11.25; HsNIK; Serine/threonine-protein kinase NIK; NF-kappa-beta-inducing kinase; Mitogen-activated protein kinase kinase kinase 14 |
Application Instructions
| Application Suggestion |
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| Application Note | Immunoblotting: 2 - 4 ug/ml. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | NIK-transfected 293 cells. |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99558 Human Mitogen-activated protein kinase kinase kinase 14 |
|---|---|
| Gene Symbol | MAP3K14 |
| Gene Full Name | mitogen-activated protein kinase kinase kinase 14 |
| Background | Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, LPS, and mitogens. A serine/threonine protein kinase which mediates NF-κB activation by IL-1, TNFα, and CD95 has been identified and designated NIK (for NF-κB inducing kinase). NIK is an activator of IκB kinase alpha and beta (IKKα and IKKβ). Therefore, NIK is a key molecule in the NF-κB signaling pathway leading to the expression of a variety of gene products in response to pro-inflammatory cytokines and bacterial products. |
| Function | Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody |
| Calculated MW | 104 kDa |
| PTM | Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization. Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. |
