ARG22747
anti-NKG2A + C + E antibody [20d5] (low endotoxin)
anti-NKG2A + C + E antibody [20d5] (low endotoxin) for Flow cytometry,Functional study and Mouse
Overview
| Product Description | Azide free and low endotoxin Rat Monoclonal antibody [20d5] recognizes NKG2A + C + E This antibody recognizes murine NKG2A, NKG2C and NKG2E, which are isoforms of the NKG2 lectin-like family. In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells. The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa-1 in the mouse (Colmenero et al. 2007). Rat anti Mouse NKG2A/C/E, clone 20d5 is reported to block ligand binding to the receptor (Vance et al. 1999). |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, FuncSt |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 20d5 |
| Isotype | IgG2a |
| Target Name | NKG2A + C + E |
| Antigen Species | Mouse |
| Immunogen | CHO transfected cells expressing the B6 allele of NKG2A. |
| Conjugation | Un-conjugated |
| Alternate Names | NK cell receptor A; CD159 antigen-like family member A; NKG2-A/NKG2-B type II integral membrane protein; CD159A; CD antigen CD159a; NKG2; NKG2A; NKG2-A/B-activating NK receptor |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Purification Note | Low endotoxin |
| Buffer | PBS. |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | KLRC1 |
|---|---|
| Gene Full Name | killer cell lectin-like receptor subfamily C, member 1 |
| Background | Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. The protein encoded by this gene belongs to the killer cell lectin-like receptor family, also called NKG2 family, which is a group of transmembrane proteins preferentially expressed in NK cells. This family of proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain. This protein forms a complex with another family member, KLRD1/CD94, and has been implicated in the recognition of the MHC class I HLA-E molecules in NK cells. The genes of NKG2 family members form a killer cell lectin-like receptor gene cluster on chromosome 12. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jan 2015] |
| Function | Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. [UniProt] |
| Calculated MW | 26 kDa |
