ARG59418
anti-NLRC4 / CARD12 antibody
anti-NLRC4 / CARD12 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse
NLRC4 Inflammasome Study antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NLRC4 / CARD12 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NLRC4 / CARD12 |
| Antigen Species | Mouse |
| Immunogen | Recombinant fusion protein corresponding to aa. 89-159 of Mouse NLRC4 (NP_001028539.1). |
| Conjugation | Un-conjugated |
| Alternate Names | CLAN; AIFEC; IPAF; CLANA; CARD12; CLANC; CLANB; CLAND; NLR family CARD domain-containing protein 4; Caspase recruitment domain-containing protein 12; Ipaf; CLR2.1; CARD, LRR, and NACHT-containing protein; CLAN1; Ice protease-activating factor; Clan protein; FCAS4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse spleen and U87 | ||||||||
| Observed Size | 116 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q3UP24 Mouse NLR family CARD domain-containing protein 4 Swiss-port # Q9NPP4 Human NLR family CARD domain-containing protein 4 |
|---|---|
| Gene Symbol | NLRC4 |
| Gene Full Name | NLR family, CARD domain containing 4 |
| Background | NLRC4 / CARD12 is a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
| Function | NLRC4 / CARD12 is a key component. of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. [UniProt] |
| Cellular Localization | Cytoplasm. Cytoplasm, cytosol. Note=Cytoplasmic filaments. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30332 NLRC4 Inflammasome Antibody Panel Related products: NLRC4 antibodies; NLRC4 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| Research Area | NLRC4 Inflammasome Study antibody |
| Calculated MW | 116 kDa |
| PTM | Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro (By similarity). [UniProt] |
