ARG55670

anti-NM23A antibody

anti-NM23A antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Overview

Product Description Mouse Monoclonal antibody recognizes NM23A
Tested Reactivity Hu, Rat
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 1172CT2.4.1.1
Isotype IgG2a
Target Name NM23A
Antigen Species Human
Immunogen Purified His-tagged Human NM23A
Conjugation Un-conjugated
Alternate Names NDP kinase A; NDPK-A; NM23; Nucleoside diphosphate kinase A; Granzyme A-activated DNase; NDKA; NBS; NM23-H1; NB; NDPKA; NDK A; Tumor metastatic process-associated protein; EC 2.7.4.6; GAAD; AWD; Metastasis inhibition factor nm23

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:25
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Jurkat

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 191575 Rat NME1

GeneID: 4830 Human NME1

Swiss-port # P15531 Human Nucleoside diphosphate kinase A

Swiss-port # Q05982 Rat Nucleoside diphosphate kinase A

Gene Symbol NME1
Gene Full Name NME/NM23 nucleoside diphosphate kinase 1
Background This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]
Function Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Note=Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA
Calculated MW 17 kDa