ARG52352

anti-NMDAR1 antibody [R1JHL]

anti-NMDAR1 antibody [R1JHL] for Immunoprecipitation,Western blot and Mouse,Rat

Neuroscience antibody

Overview

Product Description

Mouse Monoclonal antibody [R1JHL] recognizes NMDAR1

Tested Reactivity Ms, Rat
Tested Application IP, WB
Host Mouse
Clonality Monoclonal
Clone R1JHL
Isotype IgG
Target Name NMDAR1
Antigen Species Rat
Immunogen Fusion protein containing amino acids 1-564 of the NR1 subunit
Conjugation Un-conjugated
Alternate Names NMDA1; GluN1; MRD8; NMD-R1; Glutamate receptor ionotropic, NMDA 1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NR1; NMDAR1

Application Instructions

Application Note Specific for the ~120k NR1 subunit of the NMDA receptor.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Culture supernatant
Buffer PBS (pH 7.4), 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl and 1.5 mM KH2PO4.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14810 Mouse GRIN1

GeneID: 24408 Rat GRIN1

Swiss-port # P35438 Mouse Glutamate receptor ionotropic, NMDA 1

Swiss-port # P35439 Rat Glutamate receptor ionotropic, NMDA 1

Gene Symbol Grin1
Gene Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 1
Background The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
Function NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. [UniProt]
Research Area Neuroscience antibody
Calculated MW 105 kDa
PTM NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.

Clone References

The human immunodeficiency virus coat protein gp120 promotes forward trafficking and surface clustering of NMDA receptors in membrane microdomains.

ICC/IF / Rat

Xu H et al.
J Neurosci.,  (2011)

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Reduced cortical BDNF expression and aberrant memory in Carf knock-out mice.

WB / Mouse

McDowell KA et al.
J Neurosci.,  (2010)

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A neuronal role for SNAP-23 in postsynaptic glutamate receptor trafficking.

WB / Rat

Suh YH et al.
Nat Neurosci.,  (2010)

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Prion protein expression modulates neuronal copper content.

WB / Mouse

Brown DR et al.
J Neurochem.,  (2003)

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