ARG22230
anti-NMDAR2B antibody [S59-36]
anti-NMDAR2B antibody [S59-36] for ICC/IF,IHC-Whole mount,Immunohistochemistry,Immunoprecipitation,Western blot and D. melanogaster ,Human,Mouse,Rat
Neuroscience antibody; Postsynaptic Receptor antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [S59-36] recognizes NMDAR2B |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Dm |
| Tested Application | ICC/IF, IHC, IHC-Wmt, IP, WB |
| Specificity | Detects ~166kDa. No cross-reactivity against NR2A. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S59-36 |
| Isotype | IgG2b |
| Target Name | NMDAR2B |
| Antigen Species | Rat |
| Immunogen | Fusion protein around aa. 20-271 (extracellular N-terminus) of Rat NMDAR2B |
| Conjugation | Un-conjugated |
| Alternate Names | MRD6; EIEE27; NR2B; hNR3; GluN2B; NR3; N-methyl D-aspartate receptor subtype 2B; Glutamate receptor ionotropic, NMDA 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl-D-aspartate receptor subunit 3; NMDAR2B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Grin2b |
| Gene Full Name | glutamate receptor, ionotropic, N-methyl D-aspartate 2B |
| Background | N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008] |
| Function | NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity). [UniProt] |
| Cellular Localization | Cell Junction, Cell membrane, postsynaptic cell membrane, Synapse |
| Research Area | Neuroscience antibody; Postsynaptic Receptor antibody |
| Calculated MW | 166 kDa |
| PTM | Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity. |
Specific References
Myc supercompetitor cells exploit the NMDA receptor to subdue their wild-type neighbours via cell competition.
IHC-Wmt / Drosophila
