ARG52372

anti-NMDAR2C phospho (Ser1096) antibody

anti-NMDAR2C phospho (Ser1096) antibody for Western blot and Mouse,Rat

Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes NMDAR2C phospho (Ser1096)
Tested Reactivity Ms, Rat
Predict Reactivity Hu, Bov, Dog, NHuPrm
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NMDAR2C
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1096 conjugated to KLH
Conjugation Un-conjugated
Alternate Names Glutamate receptor ionotropic, NMDA 2C; NR2C; Glutamate [NMDA] receptor subunit epsilon-3; GluN2C; N-methyl D-aspartate receptor subtype 2C; NMDAR2C

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note Specific for the ~140k NR2C subunit of the NMDA receptor phosphorylated at Ser1096. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to geneRate the antibody but not by the corresponding dephospho-peptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14813 Mouse GRIN2C

GeneID: 24411 Rat GRIN2C

Swiss-port # Q00961 Rat Glutamate receptor ionotropic, NMDA 2C

Swiss-port # Q01098 Mouse Glutamate receptor ionotropic, NMDA 2C

Gene Symbol GRIN2C
Gene Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 2C
Background The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996). Phosphorylation of Ser1096 by PKB on NR2C has been recently demonstrated to regulate NMDA receptor binding to 14-3-3 (Chen & Roche 2009).
Research Area Neuroscience antibody
Calculated MW 134 kDa