ARG55310
anti-NOX2 / gp91phox antibody
anti-NOX2 / gp91phox antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Immune System antibody; Metabolism antibody
Publication2
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NOX2 / gp91phox |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NOX2 / gp91phox |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human CYBB (NP_000388.2) |
| Conjugation | Un-conjugated |
| Alternate Names | Neutrophil cytochrome b 91 kDa polypeptide; gp91-phox; NADPH oxidase 2; Heme-binding membrane glycoprotein gp91phox; 558; CGD91-phox; gp91-1; EC 1.-.-.-; Cytochrome b; GP91-1; IMD34; CGD; GP91-PHOX; AMCBX2; GP91PHOX; NOX2; Cytochrome b-245 heavy chain; p22 phagocyte B-cytochrome; p91-PHOX; Superoxide-generating NADPH oxidase heavy chain subunit; Cytochrome b558 subunit beta |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | THP-1 |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CYBB |
| Gene Full Name | cytochrome b-245, beta polypeptide |
| Background | Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008] |
| Function | Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. [UniProt] |
| Research Area | Cancer antibody; Immune System antibody; Metabolism antibody |
| Calculated MW | 65 kDa |
| PTM | Glycosylated. Phosphorylated on Ser and Thr residues. |
Specific References
Pterostilbene Interferes With Lipopolysaccharide-Induced Myocardial Injury Through Oxidative Stress and Inflammasome Pathways
WB / Mouse
Neohesperidin Protects Angiotensin II-Induced Hypertension and Vascular Remodeling.
WB / Mouse
