ARG43152
anti-NPLOC4 antibody
anti-NPLOC4 antibody for Western blot,ICC/IF and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NPLOC4 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NPLOC4 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-270 of Human NPLOC4 (NP_060391.2). |
| Conjugation | Un-conjugated |
| Alternate Names | Nuclear protein localization protein 4 homolog; NPL4; Protein NPL4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse lung | ||||||
| Observed Size | ~ 68 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P60670 Mouse Nuclear protein localization protein 4 homolog Swiss-port # Q8TAT6 Human Nuclear protein localization protein 4 homolog |
|---|---|
| Gene Symbol | NPLOC4 |
| Gene Full Name | NPL4 homolog, ubiquitin recognition factor |
| Function | The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (PubMed:26471729). [UniProt] |
| Cellular Localization | Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus. Note=Associated with the endoplasmic reticulum and nuclear. [UniProt] |
| Calculated MW | 68 kDa |
