ARG63739

anti-NPM1 / Nucleophosmin antibody

anti-NPM1 / Nucleophosmin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Nucleolar Marker antibody; GC Marker antibody; Granular Component Marker antibody

Overview

Product Description Goat Polyclonal antibody recognizes NPM1 / Nucleophosmin
Tested Reactivity Hu, Ms
Predict Reactivity Cow, Rat, Dog
Tested Application ICC/IF, IHC-P, WB
Specificity This antibody is expected to recognise both report isoforms (NP_002511.1; NP_954654.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name NPM1 / Nucleophosmin
Antigen Species Human
Immunogen C-QEAIQDLWQWRKSL
Conjugation Un-conjugated
Alternate Names NPM; Nucleolar protein NO38; B23; Nucleophosmin; Numatrin; Nucleolar phosphoprotein B23

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF10 µg/ml
IHC-P5 µg/ml
WB0.01 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18148 Mouse NPM1

GeneID: 4869 Human NPM1

Swiss-port # P06748 Human Nucleophosmin

Swiss-port # Q61937 Mouse Nucleophosmin

Background This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]
Research Area Nucleolar Marker antibody; GC Marker antibody; Granular Component Marker antibody
Calculated MW 33 kDa
PTM Acetylated at C-terminal lysine residues, thereby increasing affinity to histones.
ADP-ribosylated.
Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
Sumoylated by ARF.
May be ubiquitinated. Ubiquitination leads to proteasomal degradation.