ARG23279
anti-NQO1 antibody [A180]
anti-NQO1 antibody [A180] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Dog,Human
Overview
| Product Description | Mouse Monoclonal antibody [A180] recognizes NQO1 Mouse anti human NQO1 antibody, clone A180 specifically recognises human NQO1 (NAD(P)H:quinone oxidoreductase 1), a 31kDa cytosolic flavoenzyme and member of the NAD(P)H dehydrogenase (quinone) family, involved in detoxification, which catalyzes the two-electron reduction of quinones to hydroquinones. NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumours including lung, brain, liver, colon and breast. The ability of NQO1 to bioactivate anti-tumour quinones including mitomycin C and diaziquone, has become a focus area for chemotherapeutic studies. Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity and the NQO1 *2 allele (Nerbert, D. W. et al. 2002). Clone A180 recognizes the product of the wild-type allele NQO1*1 but does not recognize the product of the mutant NQO1*2 allele (Sato, M. et al. 2010 and Fegerholm, R. et al. 2008). Mouse anti human NQO1 antibody, clone A180 detects endogenous levels of total NQO1, and does not cross-react with NQO2 (Siegal, D. et al. 1998). Clone A180 is reported as suitable for use in immunofluorescence studies. |
|---|---|
| Tested Reactivity | Hu, Dog |
| Predict Reactivity | Ms, Rat, Mk, Rb |
| Species Does Not React With | Ms |
| Tested Application | FACS, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | A180 |
| Isotype | IgG1 |
| Target Name | NQO1 |
| Antigen Species | Human |
| Immunogen | Purified recombinant Human NQO1 |
| Conjugation | Un-conjugated |
| Alternate Names | DTD; QR1; DHQU; DIA4; NMOR1; NMORI; NAD(P)H dehydrogenase [quinone] 1; EC 1.6.5.2; Azoreductase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1; QR1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0). WB: This product detects a band of approximately 31 kDa in HeLa and HepG2 cell lysates. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NQO1 |
| Gene Full Name | NAD(P)H dehydrogenase, quinone 1 |
| Background | This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. [UniProt] |
| Highlight | Related products: NQO1 antibodies; NQO1 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Keap1-Nrf2-ARE antibody panel is launched |
| Calculated MW | 31 kDa |
