ARG63196

anti-NQO1 antibody

anti-NQO1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat,Pig

Metabolism antibody; Signaling Transduction antibody
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Overview

Product Description

Goat Polyclonal antibody recognizes NQO1

Tested Reactivity Hu, Rat, Pig
Predict Reactivity Dog
Tested Application ICC/IF, IHC-P, WB
Specificity This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name NQO1
Antigen Species Human
Immunogen C-SIPTDNQIKARK
Conjugation Un-conjugated
Alternate Names NMOR1; EC 1.6.5.2; NAD; DHQU; P; Quinone reductase 1; DT-diaphorase; DTD; QR1; Phylloquinone reductase; NMORI; DIA4; Menadione reductase; Azoreductase

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF5 - 10 µg/ml
IHC-P5 µg/ml
WB0.03 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1728 Human NQO1

GeneID: 24314 Rat NQO1

Swiss-port # P05982 Rat NAD(P)H dehydrogenase [quinone] 1

Swiss-port # P15559 Human NAD(P)H dehydrogenase [quinone] 1

Background This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Highlight Related products:
NQO1 antibodies; NQO1 Duos / Panels; Anti-Goat IgG secondary antibodies;
Related news:
Keap1-Nrf2-ARE antibody panel is launched
Research Area Metabolism antibody; Signaling Transduction antibody
Calculated MW 31 kDa

Customer's Feedback

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Review for anti-NQO1 antibody

Application:WB

Sample:MCF7

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC