ARG63196
anti-NQO1 antibody
anti-NQO1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat,Pig
Metabolism antibody; Signaling Transduction antibody
1
Overview
| Product Description | Goat Polyclonal antibody recognizes NQO1 |
|---|---|
| Tested Reactivity | Hu, Rat, Pig |
| Predict Reactivity | Dog |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NQO1 |
| Antigen Species | Human |
| Immunogen | C-SIPTDNQIKARK |
| Conjugation | Un-conjugated |
| Alternate Names | NMOR1; EC 1.6.5.2; NAD; DHQU; P; Quinone reductase 1; DT-diaphorase; DTD; QR1; Phylloquinone reductase; NMORI; DIA4; Menadione reductase; Azoreductase |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Highlight | Related products: NQO1 antibodies; NQO1 Duos / Panels; Anti-Goat IgG secondary antibodies; Related news: Keap1-Nrf2-ARE antibody panel is launched |
| Research Area | Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 31 kDa |
Customer's Feedback
Good
Review for anti-NQO1 antibody
Application：WB
Sample：MCF7
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
