ARG62512

anti-NRG1 / Heregulin beta 1 antibody [7D5]

anti-NRG1 / Heregulin beta 1 antibody [7D5] for Western blot,Immunoprecipitation,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human,Mouse,Rat

Neuroscience antibody

Overview

Product Description Mouse Monoclonal antibody [7D5] recognizes NRG1 / Heregulin beta 1
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-Fr, IHC-P, IP, WB
Specificity This antibody reacts with a 44 kD protein known as NRG1 (Neuregulin 1)/ Heregulin/Neu Differentiation Factor/NDF.
Host Mouse
Clonality Monoclonal
Clone 7D5
Isotype IgG2a
Target Name NRG1 / Heregulin beta 1
Antigen Species Rat
Immunogen Recombinant extracellular domain of rat NDF protein
Conjugation Un-conjugated
Alternate Names Sensory and motor neuron-derived factor; Heregulin; GGF2; Glial growth factor; Acetylcholine receptor-inducing activity; SMDF; ARIA; NRG1-IT2; Neu differentiation factor; HRGA; NDF; Breast cancer cell differentiation factor p45; HGL; GGF; MSTP131; Pro-NRG1; HRG; MST131; HRG1; Pro-neuregulin-1, membrane-bound isoform

Application Instructions

Application Note WB: 1-5 μg/ml
IP: 2 μl/mg of lysate
IHC: 1/10-1/500
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS (pH 7.4) and 0.08% Sodium azide
Preservative 0.08% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 112400 Rat NRG1

GeneID: 3084 Human NRG1

Swiss-port # P43322 Rat Pro-neuregulin-1, membrane-bound isoform

Swiss-port # Q02297 Human Pro-neuregulin-1, membrane-bound isoform

Gene Symbol Nrg1
Gene Full Name neuregulin 1
Background The protein encoded by this gene is a membrane glycoprotein that that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Jun 2014]
Function Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vessicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart (By similarity). [UniProt]
Cellular Localization Secreted; Cell membrane
Research Area Neuroscience antibody
Calculated MW 70 kDa
PTM Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage (By similarity).

Clone References

Neuregulin-1 (Nrg1) is mainly expressed in rat pituitary gonadotroph cells and possibly regulates prolactin (PRL) secretion in a juxtacrine manner.

WB / Rat

Zhao W et al.
J Neuroendocrinol.,  (2011)

publication_link

 

hr_line

Endothelium-derived neuregulin protects the heart against ischemic injury.

WB / Human

Hedhli N et al.
Circulation.,  (2011)

publication_link

 

hr_line

Phenotypic characterization of transgenic mice overexpressing neuregulin-1.

WB / Mouse

Kato T et al.
PLoS One.,  (2010)

publication_link

 

hr_line

Ventricular ErbB2/ErbB4 activation and downstream signaling in pacing-induced heart failure.

WB / Dog

Doggen K et al.
J Mol Cell Cardiol.,  (2009)

publication_link

 

hr_line

Peripheral neuropathy in the twitcher mouse: accumulation of extracellular matrix in the endoneurium and aberrant expression of ion channels.

IHC / Mouse

Kagitani-Shimono K et al.
Acta Neuropathol.,  (2008)

publication_link

 

hr_line

NRG1 gene rearrangements in clinical breast cancer: identification of an adjacent novel amplicon associated with poor prognosis.

IHC / Human

Prentice LM et al.
Oncogene.,  (2005)

publication_link

 

hr_line

Back signaling by the Nrg-1 intracellular domain.

ICC/IF / Mouse

Bao J et al.
J Cell Biol.,  (2003)

publication_link

 

hr_line

Reciprocal signaling between spiral ganglion neurons and Schwann cells involves neuregulin and neurotrophins.

ICC/IF / Rat

Hansen MR et al.
Hear Res.,  (2001)

publication_link

 

hr_line