ARG62565
anti-NSE / Neuron Specific Enolase antibody [5E2]
anti-NSE / Neuron Specific Enolase antibody [5E2] for ELISA,Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human
Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [5E2] recognizes NSE / Neuron Specific Enolase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5E2 |
| Isotype | IgG2a |
| Target Name | NSE / Neuron Specific Enolase |
| Antigen Species | Human |
| Immunogen | Purified full length native protein (Human) |
| Conjugation | Un-conjugated |
| Alternate Names | Neural enolase; NSE; Enolase 2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; HEL-S-279; Neuron-specific enolase; EC 4.2.1.11 |
Application Instructions
| Application Note | WB: 1/100 - 1/2000 IHC: 1/10 - 1/500 IHC-P: 1/100 - 1/300 ELISA: 1/100 - 1/2000 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | PBS (pH 7.4) and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ENO2 |
| Gene Full Name | enolase 2 (gamma, neuronal) |
| Background | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008] |
| Function | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt] |
| Research Area | Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 47 kDa |
