ARG42230

anti-NSG1 / D4S234E antibody

anti-NSG1 / D4S234E antibody for Western blot,ICC/IF and Human,Mouse,Rat

Overview

Product Description Goat Polyclonal antibody recognizes NSG1 / D4S234E
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Cow
Tested Application ICC/IF, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name NSG1 / D4S234E
Antigen Species Rat
Immunogen Synthetic peptide around the N-terminus of Rat NSG1 / D4S234E. (EKGTKQPLLEDGFD-C) (NP_077042.2)
Conjugation Un-conjugated
Alternate Names NEEP21; Neuron-specific protein family member 1; D4S234E; Brain neuron cytoplasmic protein 1; P21; D4S234

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1 - 5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Fetal Mouse brain
Observed Size ~ 21 kDa

Properties

Form Liquid
Purification Ammonium sulphate precipitation followed by affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18196 Mouse NSG1

GeneID: 25247 Rat NSG1

GeneID: 27065 Human NSG1

Gene Symbol NSG1
Gene Full Name neuron specific gene family member 1
Function Plays a role in the recycling mechanism in neurons of multiple receptors, including AMPAR, APP and L1CAM and acts at the level of early endosomes to promote sorting of receptors toward a recycling pathway. Regulates sorting and recycling of GRIA2 through interaction with GRIP1 and then contributes to the regulation of synaptic transmission and plasticity by affecting the recycling and targeting of AMPA receptors to the synapse (By similarity). Is required for faithful sorting of L1CAM to axons by facilitating trafficking from somatodendritic early endosome or the recycling endosome (By similarity). In an other hand, induces apoptosis via the activation of CASP3 in response to DNA damage (PubMed:20599942, PubMed:20878061). [UniProt]
Cellular Localization Membrane; Single-pass type II membrane protein. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Cell projection, dendrite. Early endosome membrane. Late endosome membrane. Lysosome lumen. Recycling endosome membrane. Cytoplasmic vesicle membrane. Golgi apparatus, Golgi stack membrane. Endosome, multivesicular body membrane. Endoplasmic reticulum membrane. [UniProt]
Calculated MW 21 kDa