ARG55719
anti-NSMCE2 / NSE2 antibody
anti-NSMCE2 / NSE2 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NSMCE2 / NSE2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NSMCE2 / NSE2 |
| Immunogen | Synthetic peptide (15 aa) within aa. 170-220 of Human NSMCE2 / NSE2. |
| Conjugation | Un-conjugated |
| Alternate Names | hMMS21; E3 SUMO-protein ligase NSE2; EC 6.3.2.-; NSE2; MMS21; Non-SMC element 2 homolog; C8orf36; Non-structural maintenance of chromosomes element 2 homolog; MMS21 homolog; ZMIZ7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | 293 cell lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NSMCE2 |
| Gene Full Name | NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase |
| Function | E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines. Required for sister chromatid cohesion during prometaphase and mitotic progression. [UniProt] |
| Calculated MW | 28 kDa |
| PTM | Sumoylated, possibly via autosumoylation. |
