ARG70020

anti-NT4 / Neurotrophin 5 antibody

anti-NT4 / Neurotrophin 5 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Neuroscience antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes NT4 / Neurotrophin 5
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Bov, Dog, Hrs, Pig
Tested Application ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NT4 / Neurotrophin 5
Antigen Species Human
Immunogen Synthetic peptide around the internal region of Human NT-4.
Epitope Epitope mapping within an internal region of NT-4 of human origin
Conjugation Un-conjugated
Alternate Names NTF5; NT-4/5; NT5; NT4; GLC10; GLC1O; NT-5; NT-4; Neurotrophin-4; Neurotrophin-5; Neutrophic factor 4

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:30 - 1:3000
ICC/IF1:50 - 1:500
IHC-Fr1:50 - 1:500
IHC-P1:50 - 1:500
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
WB1:100 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse skin ectract and Human colon ectract.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 1.0 ml of PBS, < 0.1% Sodium azide and 0.1% Gelatin
Preservative < 0.1% Sodium azide
Stabilizer 0.1% Gelatin
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25730 Rat NTF4

GeneID: 4909 Human NTF4

Swiss-port # P34130 Human Neurotrophin-4

Swiss-port # P34131 Rat Neurotrophin-4

Gene Symbol NTF4
Gene Full Name neurotrophin 4
Background This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood. [provided by RefSeq, Jul 2008]
Function Target-derived survival factor for peripheral sensory sympathetic neurons. [UniProt]
Research Area Neuroscience antibody
Calculated MW 22 kDa

Customer's Feedback

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Review for anti-NT4 / Neurotrophin 5 antibody

Application:WB

Sample:MCF-7

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC