ARG53957
anti-NTAL / LAB antibody [NAP-07] (PE)
anti-NTAL / LAB antibody [NAP-07] (PE) for Flow cytometry and Human,Mouse
Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [NAP-07] recognizes NTAL / LAB |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS |
| Specificity | The clone NAP-07 reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | NAP-07 |
| Isotype | IgG1 |
| Target Name | NTAL / LAB |
| Antigen Species | Human |
| Immunogen | Recombinant cytoplasmic domain (aa 91-243) of human NTAL. |
| Conjugation | PE |
| Alternate Names | Membrane-associated adapter molecule; Non-T-cell activation linker; WSCR5; WBSCR15; Linker for activation of B-cells; HSPC046; LAB; WBSCR5; NTAL; Williams-Beuren syndrome chromosomal region 15 protein; Linker for activation of T-cells family member 2; Williams-Beuren syndrome chromosomal region 5 protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9GZY6 Human Linker for activation of T-cells family member 2 Swiss-port # Q9JHL0 Mouse Linker for activation of T-cells family member 2 |
|---|---|
| Gene Symbol | LAT2 |
| Gene Full Name | linker for activation of T cells family, member 2 |
| Background | NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function. |
| Function | Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2. [UniProt] |
| Research Area | Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 27 kDa |
| PTM | Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2. May be polyubiquitinated. |
Clone References
Vav and Rac activation in B cell antigen receptor endocytosis involves Vav recruitment to the adapter protein LAB.
WB / Mouse
Regulation of Ca2+ signaling in mast cells by tyrosine-phosphorylated and unphosphorylated non-T cell activation linker.
Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells.
Comparative gene expression profiling of in vitro differentiated megakaryocytes and erythroblasts identifies novel activatory and inhibitory platelet membrane proteins.
Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor.
