ARG63278
anti-NUMB antibody
anti-NUMB antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
Product Description | Goat Polyclonal antibody recognizes NUMB |
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Tested Reactivity | Hu, Ms |
Predict Reactivity | Cow, Dog |
Tested Application | ICC/IF, IHC-P, WB |
Specificity | This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally. |
Host | Goat |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | NUMB |
Antigen Species | Human |
Immunogen | C-PFSSDLQKTFEIEL |
Conjugation | Un-conjugated |
Alternate Names | c14_5527; C14orf41; Protein S171; S171; h-Numb; Protein numb homolog |
Application Instructions
Application Suggestion |
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Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Positive Control | Mouse brain | ||||||||
Observed Size | ~ 76 kDa |
Properties
Form | Liquid |
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Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
Preservative | 0.02% Sodium azide |
Stabilizer | 0.5% BSA |
Concentration | 0.5 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Background | The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Research Area | Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
Calculated MW | 71 kDa |
PTM | Phosphorylated on Ser-276 and Ser-295 by CaMK1. Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. |