ARG44115

anti-NUP54 antibody

anti-NUP54 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal recognizes NUP54
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NUP54
Antigen Species Human
Immunogen Human NUP54 recombinant protein (Position: Q166-H493).
Conjugation Un-conjugated
Alternate Names NUP54; Nucleoporin 54; 54 KDa Nucleoporin; Nucleoporin 54kDa; Nucleoporin P54; Nucleoporin 54kD; DYT37

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.1-0.25 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 269113 Mouse NUP54

GeneID: 53371 Human NUP54

GeneID: 53372 Rat NUP54

Gene Symbol NUP54
Gene Full Name Nucleoporin 54
Background The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the phe-gly (FG) repeat-containing nucleoporin subset. Multiple alternatively spliced transcript variants have been found for this gene.
Function Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane.
Cellular Localization Membrane, Nuclear pore complex, Nucleus
Calculated MW 55 kDa
PTM Glycoprotein