ARG44115
anti-NUP54 antibody
anti-NUP54 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal recognizes NUP54 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NUP54 |
| Antigen Species | Human |
| Immunogen | Human NUP54 recombinant protein (Position: Q166-H493). |
| Conjugation | Un-conjugated |
| Alternate Names | NUP54; Nucleoporin 54; 54 KDa Nucleoporin; Nucleoporin 54kDa; Nucleoporin P54; Nucleoporin 54kD; DYT37 |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NUP54 |
| Gene Full Name | Nucleoporin 54 |
| Background | The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the phe-gly (FG) repeat-containing nucleoporin subset. Multiple alternatively spliced transcript variants have been found for this gene. |
| Function | Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane. |
| Cellular Localization | Membrane, Nuclear pore complex, Nucleus |
| Calculated MW | 55 kDa |
| PTM | Glycoprotein |
Images (6) Click the Picture to Zoom In
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ARG44115 anti-NUP54 antibody ICC/IF image
Immunofluorescence: U2OS stained with ARG44115 anti-NUP54 antibody at 5 μg/ml dilution.
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ARG44115 anti-NUP54 antibody WB image
Western blot: Hela, K562, HepG2 and A549 stained with ARG44115 anti-NUP54 antibody at 0.25 μg/ml dilution.
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ARG44115 anti-NUP54 antibody FACS image
Flow Cytometry: HeLa stained with ARG44115 anti-NUP54 antibody at 1 µg/10^6 cells dilution.
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ARG44115 anti-NUP54 antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG44115 anti-NUP54 antibody at 2 μg/ml dilution.
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ARG44115 anti-NUP54 antibody WB image
Western blot: RH35 stained with ARG44115 anti-NUP54 antibody at 0.25 μg/ml dilution.
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ARG44115 anti-NUP54 antibody WB image
Western blot: NIH/3T3 stained with ARG44115 anti-NUP54 antibody at 0.25 μg/ml dilution.
