ARG62564
anti-Nck antibody [NC-20 (20B-1H9)]
anti-Nck antibody [NC-20 (20B-1H9)] for Immunoprecipitation and Human
Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [NC-20 (20B-1H9)] recognizes Nck |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | NC-20 (20B-1H9) |
| Isotype | IgG1 |
| Target Name | Nck |
| Antigen Species | Human |
| Immunogen | Recombinant human Nck protein. |
| Conjugation | Un-conjugated |
| Alternate Names | NCK adaptor protein 1; Nck-1; NCKalpha; SH2/SH3 adaptor protein NCK-alpha; NCK; Cytoplasmic protein NCK1; nck-1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | A431 cells. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified Antibody |
| Buffer | 1X PBS and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NCK1 |
| Gene Full Name | NCK adaptor protein 1 |
| Background | The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010] |
| Function | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors. [UniProt] |
| Research Area | Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 43 kDa |
| PTM | Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB. |
