ARG24091

anti-Nectin 4 antibody

anti-Nectin 4 antibody for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Nectin 4
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Nectin 4
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide within the center region of Human Nectin 4.
Conjugation Un-conjugated
Alternate Names Nectin-4; Ig superfamily receptor LNIR; PRR4; LNIR; nectin-4; EDSS1; Poliovirus receptor-related protein 4

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
Preservative 0.01% Sodium azide
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 71740 Mouse PVRL4

GeneID: 81607 Human PVRL4

Swiss-port # Q8R007 Mouse Nectin-4

Swiss-port # Q96NY8 Human Nectin-4

Gene Symbol PVRL4
Gene Full Name poliovirus receptor-related 4
Background This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined. [provided by RefSeq, Jan 2011]
Function Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.

(Microbial infection) Acts as a receptor for measles virus. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Cell junction, adherens junction. Note=Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254). Processed poliovirus receptor-related protein 4: Secreted. Note=The secreted form is found in breast tumor patients (PubMed:15784625). [UniProt]
Calculated MW 55 kDa
PTM The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE. [UniProt]