ARG52345
anti-Nestin antibody [4D11]
anti-Nestin antibody [4D11] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
Publication3
Overview
| Product Description | Mouse Monoclonal antibody [4D11] recognizes Nestin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Chk, Cow, Hrs, Pig |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4D11 |
| Isotype | IgG1 |
| Target Name | Nestin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around aa. 317-630 of Human Nestin. |
| Conjugation | Un-conjugated |
| Alternate Names | Nestin; Nbla00170 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | Specific for the ~220-240 kDa nestin doublet in Western blot in neonatal Rat brain homogenate. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Ascites fluid |
| Buffer | Ascites fluid and 0.01 % Sodium azide |
| Preservative | 0.01 % Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NES |
| Gene Full Name | nestin |
| Background | Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells (Lendahl et al., 1990). Nestin was originally identified as a result of the production of a series of monoclonal antibodies directed against epitopes expressed on formalin fixed embryo day 15 rat spinal cord (Hockfield et al., 1985)). One of these antibodies, called Rat 401, stained fibrous profiles in the developing nervous system, but not in the mature nervous system. By screening bacteriophage expression libraries with Rat 401, Lendahl et al. were able to isolate a cDNA encoding the protein to which Rat 401 antibody bound. The protein proved to be an unusual member of the intermediate filament family, containing an alpha-helical region homologous to that found in keratin and neurofilament subunits. |
| Highlight | Related Antibody Duos and Panels: ARG30006 NSC and Astrocyte Marker Antibody Duo (GFAP, Nestin) ARG30008 Neuron Development Marker Antibody Duo (Nestin, Tuj1) ARG30009 NSC and Neuron Marker Antibody Duo (Nestin, MAP2) ARG30146 Neural Stem / Progenitor Cell Marker Antibody Duo (Nestin, Vimentin) Related products: Nestin antibodies; Nestin Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: 14-3-3η as a promising target for the treatment of Major Depression Disorder Stem cell and the regenerative medicine: Ready for the patients Neuronal Development Marker Neuronal Development Marker Choose the Best ZIKA Virus Antibodies Fight microcephaly with arigo |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody |
| Calculated MW | 177 kDa |
| PTM | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity). |
Specific References
Insulin-like growth factor reduced against decabromodiphenyl ether-209-induced neurodevelopmental toxicity in vivo and in vitro
ICC/IF / Rat
Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.
WB / Human
Transplantation of encapsulated human Leydig-like cells: A novel option for the treatment of testosterone deficiency.
ICC/IF / Human
